Polymer-based Columns for Hydrophilic Interaction Chromatography (HILIC). The Shodex™ HILICpak VG-50 is suitable for saccharides analysis and the Shodex™ HILICpak VT-50 is suitable for anionic substances analysis by HILIC mode.
Shodex™ HILICpak
Shodex™ HILICpak VG-50 Series
Features of HILICpak VG-50 Series (Recovery of Reducing Sugar)
HILICpak VG-50 4D shows high recovery ratio of reducing sugars, such as mannose. Detection of reducing sugars in low concentration is possible with VG-50. Silica-based amino column shows low recovery ratio of reducing sugars due to the interaction of the primary amino group on packing material and the carbonyl group of reducing sugar. The formation of a Schiff base causes reducing sugars to be adsorbed to the packing material.
Sample : 5mg/mL each, 5µL 1. Fructose 2. Mannose 3. Glucose 4. Sucrose
Column : Shodex HILICpak VG-50 4D (4.6mmI.D. x 150mm)
Eluent : H2O/CH3CN=20/80
Flow rate :0.6mL/min (VG-50 4D)
:1.0mL/min (Silica-based amino column)
Detector : Shodex RI
Column temp. : 40°C
Shodex™ HILICpak VT-50 for the Analysis of Phosphorylated Saccharides
This sub-series can perform high-sensitivity separations analyses of anionic samples including phosphorylated saccharides, organic acids, and other medical compounds. This product is suitable for measurement of metabolites in organisms.
