Chiral column chromatography is a variant of chromatography in which the stationary phase contains a single enantiomer of a chiral compound rather than being achiral. The two enantiomers of the same analyte compound differ in affinity to the single-enantiomer stationary phase and therefore they exit the column at different times. The chiral stationary phases consist of a suitable chiral compound attached to the surface of an achiral support such as silica gel, which creates a Chiral Stationary Phase (CSP).
